目的研究低温层积解除阜康阿魏种子休眠的规律和解除休眠过程中内源激素量的变化规律。方法种子在4℃低温层积处理,20℃培养箱发芽。高效液相色谱法进行种子内源激素Z、GA₃、IAA和ABA的测定。结果低温层积20 d时,种子发芽率为14%,40 d时种子发芽率可达60%以上。解除休眠过程中种子的内源激素量逐渐降低,在低温层积10~20 d过程中GA₃与ABA的量比值迅速增大。结论阜康阿魏种子4℃低温层积40 d解除休眠。GA₃与ABA的量比是种子休眠的关键因素。IAA和Z对种子萌发的进行有重要影响。

Objective To study the rule of breaking Ferula fukanensis seed dormancy under low-temperature and content changes of endo-hormone.Methods The seeds were treated with stratification under 4 ℃ and germinated under 20 ℃.The content of endo-hormone,such as Z,GA₃,IAA,and ABA,was mensurated by HPLC.Results The seed germination rate achieved as high as 14% in 20 d and more than 60% in 40 d.Among breaking the seed dormancy,the content of endo-hormone was decreased gradually,while the rate of GA₃ and ABA was increased quickly in 10—20 d under 4 ℃ stratification.Conclusion The stratification under 4 ℃ could break the seed dormancy.The rate of GA₃ and ABA is a pivotal factor of the seed dormancy.The endo-hormones IAA and Z have the significant effect on seed germination.

“十五”国家重大科技专项(2001BA701A60-07)