目的通过对耐盐性甘草愈伤组织再生植株研究,促进在盐碱滩人工栽培甘草技术的发展。方法诱导乌拉尔甘草Glycyrrhiza uralensis无菌苗子叶块和胚轴段在含盐培养基上脱分化均成功。逐步提高盐质量浓度诱导愈伤组织扩增,继之诱生不定芽,芽经扶壮后诱根,得到大量试管苗。结果子叶块和胚轴段在MS+BA1.5 mg/L+2.4-D 1.2 mg/L+NaCl 100 mg/L中脱分化效果好,愈伤多为淡黄色,稍透明。NaCl质量浓度增至250mg/L后愈伤组织长势很快下降,色暗,有些死亡。愈伤组织经无盐培养继代两轮后,转入M S+BA 0.5 mg/L+KT1.0 mg/L+NAA 1.0 mg/L+NaCl 200 mg/L上分化出大量不定芽,说明不定芽的耐盐性是遗传所致。降温(18~22℃)、自然光照、并添加适量的GA₃有利于壮芽形成,在诱导生根的83个芽中的55个生根,其中1/2M S+IAA1.0 mg/L+NAA 1.0 mg/L+NaCl 200 mg/L最适宜生根,生根率达66.26%。结论通过对耐盐性甘草愈伤组织的诱导可得到耐盐性甘草的再生植株。

Objective To develop the technique of artificial cultivation of Glycyrrhiza uralensis on salina by exploring regenerated plantlet from the salt-tolerant callus.Methods The experiments of dedifferentiation were successful in inducing cotyledon sections and embryonic axle sections from sterilized shoot,as explants,on salty media.The induced callus was to be multiplied and adventitious buds tobe produced when the salt concentration of medium increased gradually.Then the buds were rooting and a lot of plantlet occurring.Results Dedifferentiation of cotyledon sections and embryonic axle sections was preferable on MS+BA 1.5 mg/L+2.4-D 1.2 mg/L+NaCl 100 mg/L.Callus was hazel and just a little translucent.Growth of callus became weaker and weaker after increasing concentration of NaCl to 250 mg/L,the color got darker,and much callus died.A lot of adventitious buds came into being on MS+BA(0.5 mg/L+)KT 1.0 mg/L+NAA 1.0 mg/L+NaCl 200 mg/L after inoculating the callus on non-salty media for multiplying twice.It proved that the salt-tolerant character of the buds was induced by reason of genetics.Decreasing temperature(18—22 ℃) at natural light and adding an appropriate quantity of GA₃ in medium were helpful for the formation of strong sprouts.The rooting rate was 66.26% with rooting 55 adventitious buds among 83,and medium of 1/2 MS+IAA 1.0 mg/L+NAA 1.0 mg/L+NaCl 200 mg/L adapted to root. Conclusion A lot of salt-tolerant regenerated plantlets of G.uralensis occur after inducing the salt-tolerant callus to redifferentiate for getting the regenerated plantlet.