目的研究我国传统药用植物抗烟草花叶病毒(tobacco m osa ic v irus,TM V)的作用,为从植物中开发抗病毒类生物农药提供依据。方法采用局部枯斑法和叶碟法对我国78科162种药用植物提取物的抗TM V侵染和增殖作用进行测定。结果在10 mg/mL水平下,19种植物提取物可有效抑制TM V侵染,9种植物提取物有明显抑制TM V增殖作用。结论大多数药用植物提取物具有一定抗TM V侵染活性,仅少数药用植物提取物有较好的抗TM V增殖作用。

Objective To study antiviral activities of medicinal plant extracts against tobacco mosaic virus(TMV) and provide basis for exploitation of bio-pesticides from plant resource.Methods Using(local) lesion and leaf discs assay,extracts from 162 medicinal plant species belonging to 78 families in China were screened for antiviral activity against TMV.Results At a concentration of 10 mg/mL,extracts of 19 plant species could effectively inhibit TMV infection;nine plant extracts significantly inhibited the multiplication of TMV in infected leaf discs.Conclusion Most of the plant extracts exhibits certain antiviral activity of inhibiting TMV infection,but only a few have inhibitory effect on TMV multiplication.

福建省科技厅重点资助项目(99-Z-193);福建省教育厅项目(K04037)