目的初步探讨四物汤中糖类物质与四物汤补血作用的关系。方法四物汤部位分离采用醇沉法、萃取法和大孔吸附树脂法。用辐射小鼠血虚证模型考察活性。结果将前期发现的具有提高辐射血虚模型小鼠外周血白细胞数和促进骨髓造血祖细胞增殖作用的四物汤部位C 2(正丁醇部位),用吸附树脂法分离为C 2-1(糖部分)和C 2-2(非糖部分)两个部位。活性研究表明部位C 2-1具有提高模型小鼠外周血白细胞数和促进骨髓造血祖细胞增殖的作用,部位C 2-2仅显示促进骨髓造血祖细胞增殖的作用。按照部位C 2中糖类的组成和质量分数,将D-果糖、D-葡萄糖和蔗糖混合,活性研究表明混合糖具有促进模型小鼠骨髓造血祖细胞增殖的作用,但对外周血白细胞的作用不显著。结论单、双糖类物质对四物汤促进辐射小鼠造血功能的作用有一定贡献。

Objective To make preliminary study on the relationship between saccharides and blood-tonifing effect of Si-Wu-Tang.Methods Si-Wu-Tang was fractionated by ethanol precipitation, extraction,and macroporous adsorption resin chromatography.Activity was investigated on irradiated mice as model of blood deficiency.Results The fraction C2(n-butanol fraction),which had previously shown activity of raising the amount of peripheral leukocyte and promoting the proliferation of hematopoietic progenitor cell in bone marrow of irradiated mice,was separated into two fractions,fraction C2-1(saccharide fraction) and C2-2(non-saccharide fraction),by macroporous adsorption resin chromatography.Activity experiment showed that fraction C2-1 could raise the amount of peripheral leukocyte and promote the proliferation of hematopoietic progenitor cell in bone marrow of irradiated mice,while fraction C2-2 showed only the effect of promoting the proliferation of hematopoietic progenitor cell in bone marrow.Then,according to the composition and contents of saccharides in fraction C2,D-fructose,D-glucose,and sucrose were mixed. Activity experiment showed that the saccharide mixture could promote the proliferation of hematopoietic progenitor cell in bone marrow of irradiated mice,but the effect of raising the amount of peripheral leukocyte was not significant.Conclusion To some extents,the monosaccharide and disaccharide contribute to Si-Wu-Tang's effect of improving hematopoietic function in irradiated mice.

国家自然科学基金项目(30271617,30070913);军队“十五”重点项目(01Z019);北京市“二四八”重大创新工程项目(H010210220113)