目的尝试构建基于生物热力学表达的中药品质评价方法体系。方法以不同生长年份黄连为例,利用微量热法,测定黄连作用于大肠杆菌生长代谢过程中的热功率图,建立生物热动力学模型,分析热动力学参数、生物活性与化学成分之间的相关性。结果在不同生长年份黄连的作用下,大肠杆菌生长代谢的热功率图不尽一致,呈现较明显的指纹特征;与正常对照组比较,不同生长年份黄连均能不同程度地使大肠杆菌生长速率常数减小,传代时间延长,最大产热功率降低,说明不同生长年份黄连对大肠杆菌生长代谢均有不同程度的抑制作用,其中以含总生物碱最高的四年生黄连的抑制作用最强。结论不同生长年份黄连作用于大肠杆菌生长代谢过程的部分热动力学参数与热生物活性和化学成分之间具有显著的相关性;是具有实时、在线、微量、高效、高通量、普适性好等特点的生物热力学方法和指标,既可以间接地反映黄连的生物活性,也可作为评价中药品质的一种新方法。

Objective To establish a set of methods of Chinese materia medica quality evaluation based on biothermodynamics.Methods Using microcalorimetry,the thermal power of Escherichia coli meta-bolism affected by Rhizoma Coptidis in different growing years was measured.According to the thermokinetic model,the relationship of the thermokinetic parameters,caloric bioactivity and chemical constituent was analyzed.Results There were obvious difference in the caloric active fingerprints of E.coli metabolism affected by Rhizoma Coptidis in different growing years,which presented significant finger character.Compared with the normal groups,the metabolism of E.coli was inhibited in different levels by Rhizoma Coptidis in different growing periods,the multiplication rate constants and heat output were decreased,and the generation time was prolonged.Especially,Rhizoma Coptidis under four years' growing period with the highest content of total alkaloids showed a significant inhibitory effect on E.coli.Conclusion There are evident correlation among the thermokinetic parameters of E.coli metabolism affected by Rhizoma Coptidis in different growing years,the caloric bioactivity,and chemical composition.The thermokinetic parameters can indirectly reflect caloric bioactivity of Rhizoma Coptidis.As the method of biothermodynamics is on line,microamount,high throughout screening,and universality.The microcalorimetry is considered to be a useful mean for Chinese materia medica quality evaluation.

国家自然科学基金资助项目(3997091130371724)