目的研究青藤碱体外清除氧自由基与抗脂质过氧化的作用。结论超氧阴离子自由基(O²÷)由邻苯三酚自氧化法产生，采用F en ton体系产生羟自由基(.OH)，研究青藤碱对O²÷和.OH的清除作用;用硫代巴比妥酸(TBA)法测定VC-Fe²⁺系统诱导大鼠肝匀浆及肝线粒体产生的脂质过氧化物丙二醛(M DA)，研究青藤碱抗脂质过氧化的作用。结果青藤碱能清除.OH和O²÷，其EC50分别为1.35和0.136mg/mL，且呈浓度依赖关系，青藤碱能抑制大鼠肝匀浆自氧化及VC-Fe²⁺系统诱导引起的脂质过氧化。结论青藤碱具有清除氧自由基和抗脂质过氧化的作用，对肝线粒体也有保护作用。

Objective To study the effects of sinomenine on scavenging oxygen free radical and anti-lipid peroxidation in vitro. Methods Superoxide anion free radical(O_2~÷) induced by the auto-oxidation of pyrogallol and hydroxyl free radical(OH) induced by Fenton system were determined by spectrophotometry.The inhibition on homogenate and mitochondrial lipid peroxidation,malondialdehyde(MDA),of rat liver induced by VC-Fe(2+) system was measured by thiobarabituric acid(TBA) colorimetric method.Results Sinomenine could significantly scavenge O2~÷ and ·OH,it's EC_(50) were 1.35 and 0.136 mg/mL respectively.Sinomenine could also remarkably inhibit the lipid peroxidation of rat liver homogenate and mitochondria induced by auto-oxidation and VC-Fe(2+) system.The scavenging and inhibitory effect exhibited in a concentration-dependent manner.Conclusion The Results indicate that sinomenine possesses the effect of scavenging oxygen free radicals and anti-lipid peroxidation,which shows the protection of liver mitochondria

湖北省自然科学基金资助课题(2001ABB156)