目的研究附子中脂类生物碱(脂碱)的溶解性，确定四逆汤中脂碱的来源。结论利用电喷雾离子阱质谱分析比较生附子、四逆汤、煎煮后生附子药渣、生附子与半夏共煎液中的生物碱。结果在四逆汤中检测到少量脂碱，在生附子与半夏共煎液中未检测到脂碱，脂碱成为煎煮过生附子中的主要生物碱。结论脂碱在加热条件下也不溶于水，在四逆汤中检测到的脂碱来源于双酯型生物碱的酯交换反应。

Objective To obtain anamorphosis of Shiraia bambusicola producing the component,hypocrellin A effectively.Methods A strain was isolated from the fruit of S.bambusicola by separating its hypha and was cultured by solid-state fermentation.One of the pure chemical compounds isolated from the stroma of wild S.bambusicola was studied on the basis of UV/VIS,IR,MS spectrometric analysis and physicochemical constants.Results The pigment is proved to be hypocrellin A.The isolate was determined as the stable anamorphosis strain 0258 of S.bambusicola.Conclusion A namorphosis strain of S.bambusicola is obtained and precisely appraised for the first time.This study will provide basis for the future massively industrialized hypocrellin production.

深圳市科技计划资助项目(200528)