目的制备能适合于工业化大生产具有较高载药量的葛根芩连微丸?方法采用挤出滚圆法制备葛根芩连微丸,对影响成球的4个关键参数水分比例?挤出速度?滚圆转速和滚圆时间进行筛选优化?结果制得的葛根芩连微丸载药量达70%?圆整度好?粒径分布窄,收率在90%以上?结论采用挤出滚圆法制备高载药量的葛根芩连微丸在工艺上是可行的,对中药微丸工业化大生产具有借鉴作用?

Objective To determine the relative molecular weight（M,）of polysaceharide一2b of moutan （PSM_2bA）to establish the standard of reference substance.Methods PSM_2b was separated by a Sepharose4B column and then PSM_2bA and PSM_2bD were obtained after cryodesicca”on.Then HPI,C was used to determine their M.Ultrapure water was used as mobile phase to compare with 0.7%Na₂SO₄ solution.Results PSM_2bA was pure.The M and polydispersity（D）of PSM_2bA obtained by use of dextran as standard were 3.596 2×10⁵and 4.15 i11 0.7%Na₂SO₄ solution phase,respectively,and inul trapure water phase the M,and D were 4.758×10⁵5 and 1.03,respectively+Conelusion Different results are detected by different mobile phases in determination of Mr and D of PSM_2bA by HPI,C.And theul trapure water as mobile phase is more suitable than 0.7%Na₂SO₄ solution.

国家自然科学基金资助项目(30240066);安徽省“十五”重大科技专项基金资助项目(01803011);安徽省自然科学基金资助项目(03043301)