目的采用RP-HPLC(DAD)对不同产地毛脉酸模药材进行指纹图谱比较。方法用PlanetsilC18分析柱,甲醇-0.1%磷酸水线性梯度洗脱,检测波长254nm,洗脱时间50min,体积流量1.0mL/min,柱温35℃,测定了12个不同产地的毛脉酸模药材的HPLC指纹图谱。结果方法学考察表明,本研究建立的分析方法有较好的重现性;不同产地毛脉酸模药材共有峰面积的比值有一定的差异。结论本方法可用作毛脉酸模药材的具有专属性的指纹图谱的建立。

Objective To compare the HPLC fingerprint of Rumex gmelini from different habitats by RP-HPLC (DAD). Methods In this paper, 12 different samples were studied. Separation was performed on an Planetsil C_ 18 column, with mobile phase consisting of methanol and 0.1% phosphoric acid-water and with gradient elution at the flow rate of 1.0 mL/min. The UV detection wavelength was 254 nm, column temperature was 35 ℃, and the analysis time was 50 min. Results The Results showed that this method has a good repeatability and the ratio of common peaks'area of different samples had some difference. Conclusion This method can be used to establish the chromatographic fingerprint of R. gmelini with high specificity.

国家自然科学基金资助项目(30270156)