目的研究原花青素对肿瘤坏死因子-α(TNF-α)诱导HeLa细胞凋亡和caspase-8活化的影响。方法采用流式细胞术检测细胞凋亡,荧光检测试剂盒测定caspase-8活性,Westernblot检测caspase-8酶原表达水平。结果原花青素(5、10、20mg/L)对重组人肿瘤坏死因子-α(rhTNF-α,100μg/L)诱导的HeLa细胞凋亡均有显著的抑制作用(P<0.01),呈剂量依赖关系;原花青素(5、10、20mg/L)对rhTNF-α(100μg/L)诱导的HeLa细胞caspase-8活化均有显著抑制作用(P<0.01),呈剂量依赖关系;原花青素(5、10、20mg/L)对HeLa细胞caspase-8酶原表达无明显影响。结论原花青素抑制TNF-α诱导HeLa细胞凋亡可能与其抑制caspase-8活化有关。

Objective To investigate the effects of procyanidin on apoptosis and caspase-8 activation in HeLa cell induced by TNF-α. Methods Flow cytometry was used to analyze the apoptosis. The caspase-8 fluorescent kits were used to detect the activity of caspase-8. And the expression of caspase-8 enzymogen was examined by Western blot. Results Procyanidin (5, 10, and 20 mg/L) significantly inhibited the apoptosis of HeLa cells induced by rhTNF-α (100 μg/L) (P0.01) in a dose-dependent manner; procyanidin (5, 10, and 20 mg/L) had significantly inhibition on the caspase-8 activation of HeLa cells induced by rhTNF-α (100 μg/L) (P0.01) in a dose-dependent manner. Procyanidin (5, 10, and 20 mg/L) had no effect on the expression of caspase-8 enzymogen of HeLa cells. Conclusion The inhibition of procyanidin on apoptosis of HeLa cells induced by rhTNF-α may have relation to the inhibition of caspase-8 activation.

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