目的测定桑树资源中1-脱氧野尻霉素(DNJ)并分析其生物活性.方法以氯甲酸芴甲酯(FMOC-C l)柱前衍生RP-HPLC荧光检测法对DNJ进行分析;在酶水平测定样品的α-糖苷酶抑制曲线,在离体水平用反转肠囊实验测定样品的α-糖苷酶抑制活性.结果建立了DNJ的定量方法,并应用于桑树资源中DNJ的定量;这些中药材与DNJ对照品呈现了平行的α-糖苷酶抑制曲线,对淀粉水解和葡萄糖吸收具有较好的抑制作用.结论桑树资源可以作为α-糖苷酶抑制剂治疗糖尿病.

Objective To determine 1-deoxynojirimycin(DNJ)from mulberry resources and analyse their bioactivities.Methods Determina-tion of DNJ by derivatization with 9-fluorenylmethyl chloroformate (FMOC-C1)and followed by reversed-phase high-performance liquid chromatography(RP-HPLC)and a fluorescence detector;the inhibitory curves on a-glucosidase at the enzymatic level and the activities to a-glucosidase in vitro were observed in the everted sac experiment.Results A reliable quantitative method suitable for assays of DNJ from mulberry resources has been developed.The inhibitory curves of the extracts on a-glucosidase were parallel to those of DNJ.The extracts can inhibit hydrolyzation of starch and absorption of glucose.Conclusion The medicinal parts from mulberry resources can be used for diabetic therapy as a-glucosidase inhibitor.

"863计划"创新药物和中药现代化资助项目(2003AA2Z3510);天津市自然科学重点基金资助项目(023803811)