[关键词]
[摘要]
目的测定雷公藤属3种植物(雷公藤、昆明山海棠和黑蔓)不同群体和个体中雷公藤甲素(triptolide),为评价雷公藤药材质量和寻找雷公藤的优质种质奠定基础.方法建立雷公藤甲素HPLC测定方法,并测定了全国主要分布区25个群体91个个体的木质部和韧皮部中的雷公藤甲素.结果黑蔓的雷公藤甲素质量分数很低;雷公藤和昆明山海棠种间的雷公藤甲素差异不明显,个体质量分数木质部为1.0×10-6~5.83×10-5;韧皮部为2.3×10-6 ~1.030×10 -4.结论雷公藤和昆明山海棠不同个体雷公藤甲素质量分数最高值与最低值相差约50倍,不同居群雷公藤甲素质量分数最高值与最低值相差10多倍,不同来源的药材质量差异极大,严重影响用药的安全性;雷公藤甲素质量分数高的居群位于浙江西南部和中部、湖南新宁、贵州雷山和安徽黄山;湖南新宁、贵州雷山和浙江江山居群中有雷公藤甲素质量分数极高的个体,值得进-步研究,以寻找质量分数高的优良单株.
[Key word]
[Abstract]
Objective To determine the triptolide in different populations and individuals of three species ofTripterygiumHook.f. (Tripterygium wilfordii,T.hypoglaucum,andT.regellii),establish a foundation for evaluating the quality of species inTripterygiumHook.f.,and find good germplasm ofTripterygium Hook.f.Methods HPLC method for determining triptolide was established.The triptolide in xylem and phloem of 25 populations and 91 individuals from main distribution areas in China was determined.Results The triptolide inT.regelliiwas very low.The interspecies difference betweenT.wilfordii and T.hypoglaucum was not conspicuous.The range of triptolide of the individual was 1.0×10-6-5.83×10-5in xylem and 2.3×10-6-1.030×10-4 in phloem.Conclusion The quality of species inTripterygium Hook.f.from different resources varies significantly.It will affect seriously the security of administering drugs.The highest value of triptolide is about 50 times as the lowest value in different individuals and more than 1 0 times in different populations. The populations with higher triptolide are in the southwest and middle parts of Zhejiang Province,Xinning,Hunan Province,Leishan, Guizhou Province,and Huangshan,Anhui Province.The individuals with very high contents of triptolide exists in Xinning,Hunan Provin-ce,Leishan,Guizhou Province,and Jiangshan,Zhejiang Province populations.So further study on the three populations should be carried out to find good individuals.
[中图分类号]
[基金项目]
浙江省林业重大科研项目"木本药材高效栽培技术及有效成分提取工艺的研究"资助项目(2002A01)