目的探讨石菖蒲及其成分α-细辛醚对戊四氮(PTZ)诱发的实验性癫痫幼鼠运动行为和空间学习记忆能力的影响.方法W istar幼鼠随机分为正常对照组(NC)和致痫模型组(A)、苯巴比妥钠组(B)、石菖蒲组(C)、α-细辛醚组(D),以ip PTZ 60mg/kg建立癫痫模型,各组ig给药.动态观察各组幼鼠在斜板试验、悬吊试验、自主活动试验中行为运动的变化以及在Morris水迷宫试验中空间学习记忆能力的改变.结果A组动物的转体能力、悬吊能力、自主活动能力和空间学习记忆能力明显下降,与惊厥发作严重程度呈正相关,B组次之,与其他各组比较差异均显著(P<0.05).而C组、D组各项结果与正常对照组比较差异不显著.结论PTZ诱发的幼鼠多次惊厥发作损害其行为运动能力和空间学习记忆能力,与苯巴比妥钠相比,α-细辛醚和石菖蒲能够有效地逆转PTZ所致的损害.

Objective To explore the effect ofAcorus gramineusand its component α-asarone on the ability of the experimental you-ng rats with epilepsy induced by pentylenetetrazol(PTZ)to move and memorize.Methods Wistar young rats were randomly divided into normal control group(NC),epileptic model group(A),phenobarbital sodium group(B),A.gramineusgroup(C),and α-asarone group (D).The epileptic model was established by ip PTZ 60 mg/kg and rats in all groups were given the tested drugs by ig.Changes of move-ment in inclined plate test,over hanging test,autonomic movement test and changes of spatial learning ability and memory in Morris water maze test were observed in Wistar young rats.Results The ability of rats in groupAto writhe body,suspend body,and move automatically as well as spatial learning ability and memory were obviously decreased,which had positive correlation to the extent of eclampsia.Group B is secondary to group A.There were significant differences between the above mentioned results and the other three groups(P<0.05).But there was no obvious difference among group NC,group C,and group D.Conclusion The damage of moving and learning ability and memory function of the young rats caused by frequent epilepsy paroxysm induced by PTZ could be reve-rsed more efficiently by α-asarone and A.gramineus than phenobarbital sodium.

吉林省科委自然科学基金资助项目(20030430);吉林省中医药管理局资助项目(2004-079)