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[摘要]
目的研究高效液相色谱制备-枝蒿酮酸对照品的方法.方法原料用95%乙醇提取,浓缩成浸膏后用醋酸乙酯萃取.醋酸乙酯部位用100~200目硅胶柱色谱分离,用石油醚-醋酸乙酯(3:1)梯度洗脱,收集样品进行HPLC分离制备.结果该法所得产品用HPLC归-化法定量,质量分数大于98%.结论本方法简便,产品质量高,-枝蒿酮酸产品可用做分析方法的对照品.
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[Abstract]
Objective To establish a preparative method for rupestonic acid reference substance fromArtemisia rupestrisby HPLC. Methods After raw material being extracted with 95%ethanol,the ethanol extracts were evaporated to dryness under reduced press-ure.Then the dry powder was extracted by ethyl acetate,the ethyl acetate extract was fractionated by column chromatography on silica gel(100-200mesh)using petroleumbenzine-ethyl acetate(3:1)as the eluent.The collected fraction was purified by preparative HPLC. Results The purity of the product quantitated by normality was over 98%.Conclusion The developed method iS simple and rapid and the product of rupestonic acid can be used as reference substance.
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