目的 研究肉桂醛、柠檬醛对黄曲霉和烟曲霉细胞DNA、RNA水平的影响,以期探明其抗真菌的作用机制.方法 黄曲霉、烟曲霉接种在含有不同浓度药物的蔡氏固体培养基中,同时设不加药物为空白对照,置26.5℃恒温培养3～6d,将对照组和用药组真菌细胞进行激光扫描共聚焦显微镜观察并作细胞扫描图像分析,以对DNA、RNA进行定量和定位观察.结果 用药组DNA、RNA水平发生不同变化,出现多核分生孢子.结论 肉桂醛、柠檬醛直接或间接影响了真菌细胞遗传物质的正常合成,以至不能完成正常细胞周期,影响分生孢子梗的正常分化,从而抑制真菌生长、繁殖.

Objective To investigate the effect s of cinnamaldehyde and citral on DNA and RNA of Aspergillus flavus and A. fumigatus cells and their mechanisms. Methods A. flavus and A. fumigatus were incubated on Czapek's agar plate(treated with cinnamaldehyde and citral at different concent rations) at 26.5 ℃ for 3-6d. The normal and treated cells were observed by laser scanning confocal microscope(LSCM) and image analysis to describe the DNA and RNA levels by quantity and localizationResults DNA and RNA levels were changed greatly and multinucleate coniospores appeared in the treated cellsConclusion Cinnamaldehyde and citral have directly or indirectly in terfered the conventional synthesis of fungal hereditary DNA and RNA and normal differentiation of conidiophore in A. flavus and A. fumigatus, thus inhibiting the normal cell cycle and the growth and propagation of fungi.

国家自然科学基金资助项目(30160099)