目的 观察川芎嗪(TMP)含药血清对人肝癌细胞HepG2增殖的影响.方法 选用SD雄性大鼠30只,随机分成3组,分别ipTMP143.0、71.5mg/kg、生理盐水0.8mL,制备TMP大、小剂量组及生理盐水组含药血清,采用RP-HPLC法测定含药血清中TMP的质量浓度.将各组含药血清作用于人肝癌细胞HepG248h,MTT法测定不同质量浓度TMP含药血清对人肝癌细胞HepG2增殖的抑制作用.结果 TMP大剂量组血清(20%、10%、5%),TMP小剂量组血清(20%、10%)对人肝癌细胞HepG2增殖较生理盐水组有显著抑制作用(P<0.05),最高抑制率可达46.1%.结论 TMP含药血清对人肝癌细胞HepG2增殖有一定的抑制作用.

Objective To observe the effects of rat tetramethylpyrazine(TMP)-containing serum OD the proliferation of human liver cancer cells Hep G₂Methods Thirty SD male rats were divided into three groups randomly and the serum was collected after ip TMP with the dosage of 143.0,71.5 mg／kg or NS O.8 mL to prepare the TMP-containing serum in large-and small-dose groups and NS group as well.A reversed-phase high performance liquid chromatography(RP-HPLC)method was used to determine the TMP concentration in rat serum.Human liver cancer cells Hep G₂ was treated with rat TMP-containing serum for 48 h.Inhibition of the TMP-containing serum on proliferation of Hep G2 was detected by MTT assayResults The serum of large dose TMP(20％,10％,and 5％)and small dose TMP(20％and10％)could obviously inhibit Hep G2 multiplication(PConclusion The TMP-containing serum can inhibit the proliferation of human liver cancer cells Hep G
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国家自然科学基金资助项目(30070915)