目的 建立灌胃当归胡椒复方小鼠的血浆中阿魏酸的分析方法.方法 采用HPLC法,以甲醇-水-冰醋酸(36.4:63:0.6)为流动相,色谱柱KromasilC18(250mm×4.6mm,7μm)为固定相,体积流量1.0mL/min,紫外检测波长322nm.采用外标法定量.结果 阿魏酸与血浆中其他组分能很好分离,阿魏酸在1.88～188.00ng/μL线性关系良好(r=0.999),最低检测浓度0.47ng/μL,平均回收率为94.85%.结论 本方法具有准确、灵敏、专一性高等特点,适于阿魏酸的血药浓度测定及药动学研究.

Objective To establish an analytical method for determination of ferulic acid in Danggui Hujiao(Radix Angelicae Sinensis and Fructus Piperis)Decoction in mouse plasmaMethods HPLC method was used.The conditions of chromatography：Kromasil C₁₈(250 mm×4.6 mm,7μm)was used with a mobile phase of CH₃0H-H₂0-CH₃COOH(36.4：63：O.6).Flow rate was 1.0 mL／min.The detecting wavelength was 322 nm.External standard method was quantitative analysis methodResults The ferulic acid could be totally separated from other ingredients in plasma.The linear range was 1.88-188·00 ng／pL(r=0.999),the lowest detectability was 0.47 ng／pL.and the average recovery was 94.85％Conclusion This method provides an accurate and sensitive way in detecting blood concentration of ferulic acid and studying in pharmacokinetics.

国家中医药管理局科研基金资助项目(02-03ZP50);四川省中医药管理局科研基金资助项目(200217)