目的研究金丝桃属植物短柱金丝桃Hypericumhookerianum的化学成分,并通过药理实验寻找活性成分。方法在细胞毒筛选结果指导下,同步对短柱金丝桃的活性部位进行化学成分分离纯化。经多次柱色谱、重结晶,得到纯化合物;并通过理化数据测定及波谱分析,鉴定化合物结构。结果从短柱金丝桃的有效部位中分离鉴定出7种化合物,分别是:槲皮素(quercetin,)、木犀草素(luteolin,)、芹菜素(apigenin,)、金丝桃苷(hyperin,)、黄芪苷(astragalin,)、螺内酯类化合物:金丝桃内酯A(hyperolactoneA,)和金丝桃内酯C(hyperolactoneC,)。结论上述化合物均为首次从该植物中分离得到,化合物和为首次从该属植物中分离得到;通过细胞毒试验发现,短柱金丝桃的氯仿及醋酸乙酯提取物、槲皮素及木犀草素具有细胞毒性。

Objective To study the chemical constituents in plants of Hypericum hookerianum and their activities by pharmacological experiment. Methods Cytotoxicity screening of the extracts from H. hookerianum were carried to isolate and purify the chemical constituents. The chemical structures were (identified) by physicochemical properties and spectral data analysis. Results Seven compounds were isola-(ted) and (established) as quercetin (Ⅰ), luteolin (Ⅱ), apigenin (Ⅲ), hyperin (Ⅳ), astragalin (Ⅴ), hyper-(olactone) A (Ⅵ), and hyperolactone C (Ⅶ). Conclusion All the compounds are isolated from H. hookerianum for the first time. Compounds Ⅲ and Ⅴ are isolated from the plants of Hypericum Linn. for the first time. In addition, the fractions by chlorform and ethylacete extracting, quercetin, and luteolin possess the cytotoxicity.

云南省应用科学基金(1999B004M);云南省国际合作项目(GH2001121)