目的 用 RAPD技术对红景天属(Rhodiola L.)的大花红景天 R.crenulata、长鞭红景天 R.fastigiata、狭叶红景天 R.kirilowii和高山红景天 R.sachalinensis 4种药用植物进行分子水平鉴定。方法 CATB法提取红景天属原植物总 DNA,以 200条随机引物进行随机扩增。结果 用 OPA4(AATCGGGCTG)、OPB7(GGTGACGCAG)、OPB18(CCACAGCAGT)、OPX14(ACAGGTGCTG)和 OPZ13(GACTAAGCCC)作随机扩增引物进行随机扩增时,可得到识别这些物种基因组 DNA的多态片段。结论 RAPD法能有效地鉴别红景天类药材,把大花红景天、长鞭红景天、狭叶红景天和高山红景天区分开。

Object To identify the crude drugs of Rhodiola L.,including R1 crenulata,R.fastig iata R.kirilowii,and R.saccharinensis by RAPD.Methods General DNA was isolated from fresh leaves of the four species in R hod iola L.by CTAB and the random amplified polymorphic DNA (RAPD) was used to identify them.Results The polymorphic fragment of DNA fingerprints of different species was compared by primers OPA 4 ( AATCGGGCTG),OPB7 ( GGTGACGCAG),OPB18,OPX14 ( ACA GGTGCTG ),and OPZ13 ( GACTAA GCCC ).The studies showed that they have different relationships in heredity and themethod of RA PD can be used to identify them easily.Conclusion The raw medicinalma-tericals Rhodiola L.can be identified by RAPD,and the above-mentioned four species are distingushed.