目的 考察影响二苯乙烯苷(THSG)溶液稳定性的因素,并计算室温下其晶体粉末的t0.9值.方法 采用HPLC法和HPLC-MS联用法.结果 以Lichrospher 5-C18(250 mm×4.6 mm,5μm)为固定相,甲醇-水(4:6)为流动相,检测波长为310 nm.THSG进样量在0.14～2.80μg与峰面积线性关系良好,自制THSG纯度高达99.94%,pH 5.8时,THSG与水分子发生加成反应.强酸促使THSG水解为葡萄糖和苷元,并进一步降解为酚类化合物.强碱性条件下,THSG氧化为醌类化合物.在中性及弱碱性水溶液中较稳定,30 d内含量无明显变化.THSG晶体粉末室温下放置240 d含量无明显变化,t0.9值为1 098 d.结论 二苯乙烯苷在一定条件下是稳定的。

Object To study the factorswhich influenced the stability of stilbene glucosidel(tetrahy-droxystilbene-glucoside,THSG) solution,investigate and calculate the value of _to.9 of THSG crystalpowder at room temperature·Methods HPLC and HPLC-MS Methods were used.Results The best chromatographic conditionswere to employLichrospher 5-C₁₈ (250 mm X4.6mm，5 μm)as the stable phase,methanohvater (4:6) as mobile phase,310 nm as the detective w avelength·W ithin the range from 0.14to 2.80 μg,there w as a good linearity·The content of self-prepared THSG was up to 99·94%.Stability test show ed that w hen pH w as 5.8,an additional reaction occu rred betty een TH SG and H₂O·Strong acids precipitated the hydrolyzation of THSG into glucose and aglycone,which then degraded into phenol compound.W hen strong bases existed,THSG was easily oxidized into quinine compound.Whereas itwas stable in a neutralorweak basic condition,the contentof THSG had not changed in 30 d.So was the THSG's crystalpowder stored at room temperature for 240d.The value of _t0.9 was 1 098 d.Conclusion THSG is stable in some certain conditions.