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[摘要]
目的 从DNA分子水平上分析新疆药用桑树资源9个栽培群体的遗传关系。方法 从100个随机引物中筛选出10个引物,用这10个引物做RAPD扩增,应用NTSYS软件对扩增结果 进行聚类分析。结果 共扩增出108个位点,其中多态性位点91个,多态比例为84.26%,各群体间存在较明显的遗传分化。另外,获得药桑的8个特异位点。结论 RAPD分析结果 与新疆药用桑树资源植物的遗传关系的传统划分是基本一致的。
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[Abstract]
Object To study the inherited relationships among nine medicinal mulberry populations cultivated in different regions of Xinjiang at DNA level.Methods DNA of all populations was amplified by ten random primers from 100 ramdom primers,the polymorphic bands of RA PD were counted.And the Results of populations were analyzed by clustering analysis NTSYS-PC software.Results A total of 108 bands was obtained,91 of total bands (84.26%)was polymorphic.The Results indicated that there were apparent and abundant genetic variations in Morus L.of Xinjiang.Besides,eight specific bands of M.nigra were obtained.Conclusion The genetic relationship among medicinal resources plants of mulberry in Xinjiang indicats by the RA PD analysis to be consistentw ith their traditional classifications in themain.
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