目的 比较不同产地各种菊花栽培品种的HPLC指纹特征,为构建福田河白菊的HPLC指纹图谱奠定基础。方法 采用INERTSILC18柱,乙腈-水(含2%四氢呋喃,0.1%三氟醋酸)为流动相,梯度洗脱,体积流量1mL/min;柱温30℃,对不同菊花栽培品种以及同属其他相关种进行对比色谱分析。结果 构建了菊花的HPLC指纹图谱模式,该模式显示不同产地菊花品种的HPLC图谱具有较高的相似性,有15个共有峰,其中4个为10强峰,2个为10强峰或次强峰。指纹数据反映了各品种菊花在地域和亲缘关系上的联系以及各自独特的特征。结论 该色谱系统可以用于构建福田河白菊、湖北金菊等不同菊花品种的指纹图谱。

Object To compare the HPLC fingerprints of Flos Chrysanthem i from different habitats.Methods With Inertsil C18 column,gradient elute was performed by mobil phase containing acetonitrile.water(2% tetrahydrofuran,0.1% trifluoracetic acid).The flow rate was.mL/min and column temperature 30℃.D ifferent breeds of F los Chrysanthem i from various producing areas and other species from the same genus were comparatively analyzed.Results It is discovered that the HPLC fingerprints from different breeds of F los Chrysanthem i have a strong comparability.They have 16 mutual peaks and among them four are from the ten strongest peaks,two from the ten strongest peaks or stronger peaks.The fingerprint data reflect the affiliation on producing area and genetic relationship of differnt breeds of Flos Chrysanthem i,and also characteristic features of each breed.Conclusion This method can be used to establish the HPLC fingerprints of different breeds produced in different areas.

湖北省科技攻关计划重大项目《湖北省地道药材规范化种植及示范基地建设研究》2001AA304A菊花子项目