[关键词]
[摘要]
目的 研究山茱萸免疫抑制活性部位(鞣质活性部位,F-1C)对佐剂性关节炎(AA)大鼠免疫功能的影响。方法 运用淋巴细胞增殖反应、流式细胞技术和酶联免疫吸附试验(ELISA)观察F-1C体内和体外对AA大鼠免疫功能的影响,并与环孢素A(CsA)和雷公藤多苷片(TW)进行比较。结果 ig给予F-1C(30mg/kg)对AA大鼠原发性足肿胀具有明显的治疗作用,该作用较TW强,较CsA弱;F-1C对AA大鼠低下的脾细胞增殖反应具有改善作用;对亢进的胸腺细胞增殖反应具有抑制作用。F-1C体外对AA大鼠低下的脾细胞产生IgG水平具有明显的促进作用,并能抑制亢进的胸腺细胞增殖反应。结论 F-1C能够治疗AA大鼠原发足肿胀,该作用可能与纠正AA大鼠的异常的免疫反应有关。
[Key word]
[Abstract]
Object To study the therapeutic effect and the possible mechanism of F-1C,a tannin fraction w ith immunosuppressive effect isolated from Cornus of f icinalis Sieb.et Zucc.,in the treatment of adjuvant arthritis(AA)rats.Methods AA rat model was reproduced to observe the effect on immune function both in vivo and in vitro and compare with Cyclosporine(CsA)and Trip teryg ium w ilf ord ii(TW).The effect of F-1C on joint swelling and the immune respones of AA rats were evaluated by lymphocytotic reaction,flow cytometry,and EL ISA.Results F-1C by ig adm inistration to AA rats decreased the primary joint swelling of AA rats,stronger than TW and weaker than CsA,promoted the decreased splenocyte proliferation induced by Con A,and inhibited the augmented thymocyte proliferation induced by Con A in AA rats.The evaluation in vitro showed the F-1C inhibited the augmented thymocyte proliferation from AA rats and promoted the deficient IgG produced by splenocyte from AA rats.Conclusion F-1C has therapeutic effect on AA rats and the modulating immune function is one possible underlying mechanism.
[中图分类号]
[基金项目]
国家重点基础研究发展规划项目资助(G1999054401)
