目的 建立HPLC法测定鸦胆子油中的脂肪酸的方法 。方法 以2,4′-二溴苯乙酮为衍生化试剂,18-冠-6醚为相转移催化剂,采用KromasilC8(250mm×4mm,5μm)反相柱,波长254nm,以乙腈-水(80∶20)为流动相等度洗脱,柱温室温,体积流量为1.3mL/min,正十七烷酸为内标,一次基线分离5种脂肪酸。结果 亚油酸的线性范围为0.022～0.330μg,软脂酸的线性范围为0.014～0.213μg,油酸线性范围为0.028～0.416μg,硬脂酸的线性范围为0.012～0.177μg。平均回收率分别为99.2%、97.2%、101.8%、97.8%,RSD分别为1.2%、1.5%、0.4%、2.3%。结论 该方法 重现性好,定量准确,可作为鸦胆子油中脂肪酸的定量方法 。

Object A method was developed for the determ ination of fatty acid in F ructus B ruceae oil by precolumn derivation HPLC.Methods Fatty acids were derivatized w ith p-bromophenacylbrom ide as derivative and 18-crown-6 as catalyst.The method used C8 Kromasil C8(250mm×4mm,5μm)column and isocratic acetonitrile-water eluent and the internal standard was heptadecanoic acid.The detection wavelenghth was 254 nm.Column temperature was fixed at room temperature,and the flow rate was 113mL/m in.Results The standard curves of linoleic,palm itic,oleic,and steraric acid are linear w ithin the range of 0.022—0.330,0.014—0.213,0.028—0.416,and 0.012—0.177μg,respectively.The four fatty acid recoveries are 99.2%,97.2%,101.8%,97.8%,and the RSD are 1.2%,1.5%,0.4%,2.3%,individuslly.Conclusion Five fatty acids are separated w ithin 30 m inutes during a single run.The present method is reliable and relatively simple for the determ ination of fatty acid in F ruetus B ruceae oil.

辽宁省重大课题项目(2002226005)