目的 分析不同产地泽泻中氨基酸含量及其特征性。方法 采用邻苯二甲醛 (OPA)、氯甲酸芴甲酯(FMOC)联合柱前衍生RP HPLC测定泽泻中的 17种氨基酸。结果 氨基酸质量浓度与峰面积呈良好的线性关系,r均在 0.99以上。不同产地泽泻中氨基酸含量有一定差别。结论 该方法适宜泽泻药材中氨基酸含量的测定。

Object To determine the contents of amino acids a nd study the characters of Alisma orientale (Sam. Juzep.) from d ifferent habitats. Methods The samples of A. orientale from thr ee different areas were derived with o-phthaldialdehyde (OPA) and 9-fluorenylmethyl chloroformate (FMOC) in pre-column, and separated by RP- HPLC. Seventeen kinds of amino aci ds were determined. Results The concentration of amino acids and peak areas achieve d a nice linear relations (r is higher than 0.99). There were differences of the amino acid in A. orientale from diffe rent habitats. Conclusion This method is suitable for the determination of am ino acid in A. orientale.

国家自然科学基金 2 0 0 3重点项目 ( 2 0 2 3 5 0 2 0 );福建省泽泻GAP研究项目 ( 2 0 0 2Y0 2 4)