目的 探索喜树的人工快繁。方法 以喜树幼苗的茎尖作为外植体 ,培养在附加不同激素的培养基上。结果 以 B5培养基附加 6 - BA 0 .2 m g/ L ,IBA 0 .0 5 mg/ L和 AS (afenine sulfate,10 mg/ L )对丛生芽的诱导与增值效果最佳 ,而附加 IBA0 .5 mg/ L,KT0 .1mg/ L 和 AS10 m g/ L 的生根效果最佳。试管苗移栽到珍珠岩 -土壤(3: 7)的基质中生长良好 ,成活率高达 96 %。结论 为喜树的开发利用提供了一条新途径。

Object To explore the artificial propagation of the medicinal plant, Camptotheca acuminata Decne. Methods The shoot-tip was tested as the explants and cultured on culture media with different portions of hormone.Results The best medium for bud induction was the B5 basic medium with additions of 6-BA (0.2 mg/L), IBA (0.05 mg/L), and AS (afenine sulfate, 10 mg/L). While B5 with the additions of IBA (0.5 mg/L), KT (0.1 mg/L), and AS (10 mg/L) was suitable for rooting. The seedling was cultivated and grew well on the base material mixed with perlite-soil (3:7). The survival rate of transplant was up to 96%. Conclusion The above mentioned method provides a new effective way to exploit this plant resources.

贵州省科学技术基金资助(20023033);贵州省科技厅专项基金(黔科合字 (2003)JN012)