目的 了解肉苁蓉花粉在自然条件下的寿命。方法 采用花粉离体萌发法 ,对自然生长的肉苁蓉花粉生活力进行测定研究。结果 肉苁蓉花粉萌发的最佳条件为初花期花粉 ,在恒温 2 5℃ ,以 0 .6 %琼脂 +10 %蔗糖 +0 .1%硼酸为离体培养基进行培养 ,有利于花粉的萌发和花粉管的生长 ;低温 (4℃ )条件下可进行短期的花粉贮藏 ,延长花粉的寿命。结论 为采用肉苁蓉花粉进行优良种质资源的保存和人工育种工作提供了理论依据

Object To understand pollen life of Cistanche deserticola Y.C.Ma in natural conditions. Methods To determine pollen viability of C. deserticola in natural state by pollen germination method in vitro.Results The optimum conditions of C. deserticolapollen germination are pollens in primary flowering time and a culture medium with 0.6% agar + 10% sucrose + 0.1% boric acid, cultured in 25 ℃, which is benefit to pollen germination and pollen tube growth; low temperature (4 ℃) is benefit to pollen short-dated storage and can prolong pollen life. Conclusion The study provides a theoretical basis for conserving better seed resources and artificial cultivating by pollen of C. deserticola.

国家自然科学基金项目(30260008);宁夏自然科学基金项目(C123)