目的 研究肉苁蓉总苷 (GCs)对大鼠局灶性脑缺血损伤的保护作用。方法 采用插线法阻塞大鼠大脑中动脉 (MCAO)制造大鼠局灶性脑缺血模型 ,采用 NBT染色测定脑梗死范围 ,用“重量求面积法”计算梗死组织占对侧大脑质量的百分比作为对梗死范围的判定 ,行为指标评分采用 11分评分制 ,并测定缺血脑组织的抗氧化酶活性及丙二醛 (MDA )含量。结果 GCs 12 5 ,2 5 0 m g/ kg可明显缩小脑梗死范围 ,改善神经症状 ,脑组织超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH- Px)的活性明显升高 ,MDA含量显著下降。结论 GCs对大鼠局灶性脑缺血损伤具有神经保护作用 ,此作用可能与 SOD,GSH- Px活性水平的升高有关

Object To study the protective effects of glycosides of cistanche (GCs) on focal cerebral ischemic rats. Methods Focal cerebral ischemia in rats was induced by 24 h occlusion of the middle cerebral artery (MCAO). The infarct area was measured by nitrobenzene thiocyanate (NBT) staining technique. The content of neurological deficits was evaluated by 0-11 scales. The activities of antioxidases and contents of MDA in ischemic brain tissue were analyzed. Results Significant decrease in infarct area and improvement in neurological deficits were observed by oral administration of GCs 125, 250 mg/kg, respectively. Significant increase in activities of SOD and GSH-Px, and significant decrease in contents of MDA in rats were also observed after 24 h MCAO. Conclusion GCs has a neuroprotective effect against focal cerebral ischemia and the effect may be related to the increase in activities of SOD and GSH-Px.

新疆维吾尔自治区自然科学基金资助项目(200121112)