目的 探讨不同产地牛膝的 ITS的序列变异,为鉴别牛膝提供 DNA分子标记。方法 利用特异性 PCR技术对牛膝的 r DNA ITS区碱基序列进行测定,报道了牛膝的 r DNA ITS区的碱基序列。结果 得到核糖体 DNA中的 ITS及 5.8S r DNA完全序列,18S和 2 6 S r DNA部分序列,共约 6 5 0 bp。牛膝与川牛膝及土牛膝碱基序列有明显差异,不同产地、不同栽培品种牛膝碱基序列无差异。结论 此法可用于牛膝种间及真伪品鉴别。

Object To study the correlation between TTS sequence variation of root ofA chyratcthes bidetctata Blume from different habitat to provide their DNA molecular marker for identification bidetcto to comp1eted Methods To determine rDNA TTS base sequence by peculiar PCR technology. Results The completed sequence of TTS and 5.8 S iDNA，and the partial sequence of 18S rDNA and 26 S rDNA of root of A. bidentata were obtained. They are about 650 bp. There is obvious diversity among the roots of A.bidenta ta, Cyathula of ficinalisKuan, and A. aspera, while there is no difference among the roots of A.bidentata from different habitat and by different cultivacation breeds. Conclusion The method can be used to identify the root of A. bidentata among different species and to differen tiate its fakes.

“九五”国家科技攻关项目(99-929-01-06)