目的 探讨杜仲原生皮与再生皮的次生代谢物含量的差异性。方法 利用游标卡尺测量 17龄杜仲树的原生皮与再生皮的厚度,采用分光光度法测定桃叶珊瑚苷和总黄酮含量,采用高效液相色谱法测定绿原酸含量。结果杜仲原生皮和再生皮的内皮厚度分别为1.6 4 6和1.6 31mm;原生皮中绿原酸、桃叶珊瑚苷和总黄酮的含量分别为0.4 0 2 %,0.781%和 0.4 84 %,再生皮中绿原酸、桃叶珊瑚苷和总黄酮的含量分别为 0.4 36 %,0.84 9%和 0.5 16 %。结论 原生皮与再生皮的厚度基本相同;原生皮与再生皮的次生代谢物含量无显著差异。

Object To investigate the content differences of secondary metabolites between Eucommia ulmoides Oliver original and reborn barks. Methods To measure the thickness of 17 years bark by vernier caliper, determine aucubin and flavonoids by spectrophotometer, and chlorogenic acid by HPLC. Results The thickness of original inner bark is1.646 mm, and of reborn inner bark is1.631 mm; the contents of chlorogenic acid, aucubin and flavonoids in original bark are: 0.402%, 0.781%, and 0.484%, and in reborn bark are: 0.436%, 0.849%, and 0.516% separately. Conclusion The thickness, as well as the contents of secondary metabolites of original and reborn barks are similar each other.

陕西省重大科技项目97K04-G2