目的 建立测定龙血竭中对羟基苯甲酸乙酯含量的方法。方法 以 C1 8反相键合硅胶为固定相,乙腈-1%冰醋酸 (31∶ 6 9)为流动相,检测波长为 2 5 7nm,用外标法定量。结果 对羟基苯甲酸乙酯在 0.2 0 6～ 2.884 ng有良好线性关系,r=0.9998,平均回收率为 96.0 2 %,RSD为1.87%。结论 本方法是测定龙血竭中对羟基苯甲酸乙酯含量的快速、简便、准确可靠的定量方法。

Object To establish a HPLC method for determination of ethyl p hydroxybenzoate in Dragon's Blood. Methods Using C 18 reverse phase column and acetonitrile 1% acetic acid (31∶69) as mobile phase, detecting at 257 nm and quantitating with external standard method. Results The standard curves of ethyl p hydroxybenzoate showed good linearity over the range of 0.206-2.884 ng, r=0.999 8. The average recovery was 96.02% and RSD was1.87%. Conclusion The method is quick and simple, and it is accurate and reliable for the determination of ethyl p hydroxybenzoate in Dragon's Blood.

国家自然科学基金资助项目(30070088)