目的 研究制备型高效液相色谱分离纯化茶氨酸对照品的方法。方法 原料水提后的水相用被水饱和的氯仿萃取,水相蒸发浓缩后离心,上清液进行 HPL C分离制备,收集液冷冻干燥,粗产品用甲醇清洗即得。结果 该法所得产品用外标法定量,纯度大于 98%,制备收率大于 6 0 %。结论 本法生产周期短,产品质量高,方法简便,生产费用低,茶氨酸产品可用作分析方法的对照品。

Object To establish an isolation method of theanine reference substance by preparative HPLC. Methods After raw material extracted by water, the water phase was extracted by chloroform saturated with water, then concentrated on a thermostat. After centrifuged, supernatant was isolated and purified by preparative HPLC. Fraction was frozen and dried by Flexi Drier. Crude product was rinsed by methanol. The purity of product was determined by analytical HPLC. Results The purity of product is higher than 98% and theanine yield from raw material by this method exceeds 60%. Conclusion The developed method is simple, rapid, and at low production cost. The product owns the quality of reference substance.

国家“十五”攻关资助项目(2001BA746C;2001BA804A21)