目的 建立赤芍的 HPL C指纹图谱,为科学评价与有效控制赤芍质量提供新方法。方法 采用 HPL C法,测定了 18个不同产地赤芍样品。色谱条件 :Hypersil C1 8柱 (2 0 0 mm× 4.6 mm 5 μm),流动相为乙腈 - 0.0 2 5 m ol/L H3PO4 -四氢呋喃 (9.5∶ 90.5∶ 1.2 5 ),流速 0.8m L/min,检测波长 2 5 4 nm,柱温为室温。结果 本研究建立的分析方法有较好的重复性,不同产地赤芍药材色谱峰面积比值有一定的差异。结论 色谱指纹图谱分析法能简便快速地区分不同来源的赤芍药材,为全面控制赤芍药材的质量提供了依据

Object To study the HPLC fingerprints and establish a sensitive and specific method for controlling the quality of Radix Paeoniae Rubra (RPR) methods The HPLC method was applied for quality assessment of RPR All 18 samples collected from different places were determined A Hypersil C 18 column (200 mm×4 6 mm, 5 μm) was used with the mobile phase being acetonitril 0 025 mol/L H 3PO 4: THF (9 5∶90 5∶1 25), flow rate being 0 8 mL/min, detecting wavelength being 254 nm, the column temperature being at room temperture Results This method had a good repeatability and reproducibility, and the ratio of peaks' area of different habitat samples were different Conclusion The method is suitable to differentiate RPR form different sources conveniently, and can be used as a quality control method for this herb