目的 以镇咳、祛痰、平喘为药效学指标,跟踪湖北贝母活性部位。方法 以不同溶剂提取湖北贝母浸膏,经药理筛选得到最佳提取溶剂的组分。再分别以酸碱法和溶剂法对最佳提取溶剂的组分浸膏进行分离,得到活性部位。结果 5 0 %乙醇是最好的提取溶剂 ;综合分析不同部位的镇咳、祛痰、平喘活性实验结果,样品 C即酸碱法的氯仿部位 (主要含生物碱 )效果较好。结论 酸碱法的氯仿部位 (即总生物碱 )是其镇咳、祛痰、平喘综合活性较好的部位,实验结果与文献关于贝母呼吸系统活性在生物碱部位的报道相一致

Object To track the effective part of antitussive, expectorant and antiasthmatic activities of Fritillaria hupehensi Hsiao et K.C. Hsia. methods Different solvents were used to extract the Beimu powder, and to get the extraction of the best solvent according to the activities. After that, the extraction was isolated with the acid base method and solvent method, to obtain the effective part. Results Ethanol 50% is the best extracting solvent. The third sample CHCl 3 part of acid base method (mainly containing alkaloids) is better according to the antitussive, expectorant and antiasthmatic activities. Conclusion CHCl 3 part of acid base method (mainly containing alkaloids) is the most effective part of antitussive, expectorant and antiasthmatic. This result is coincidence with the literature that the active part of respiratory system of the plants of Fritillaria L. is the alkaloid part.

湖北省科技厅重大社会发展基金项目(972P1102)