目的建立姜黄挥发油毛细管气相色谱指纹谱数据的标准化和数字化表征方法。方法在不同色谱条件下进行GC指纹谱测定,以系列正构烷烃为参比,按多项式回归分析计算各谱峰的保留指数。结果对8主峰的分析结果表明,各峰保留指数的重复性比相对于基峰的相对保留值更优越。结论采用多参比指标建立的指纹谱数字化标准,对中药质量控制更科学有效

Object To establish the standardization and digitization methods for gas chromatographic fingerprint chromatograms of the essential oil of Curcuma longa L.Methods A polynomial regression analysis technique was established for the calculation and predicti on of the gas chromatographic retention indices by using a series of normal aliphatic hydrocarbons as the reference standards.And it was used for the characterization of the features of the gas chromatographic fingerprint spectra of the essential oil of C.longa.Results It was approved that retention indices of the gas chromatographic fingerprint spectra obtained at a variety of conditions were stable and reliable with excellent reproducibility,and fairly good ruggedness.It was also much better than the relative retention time indices.Conclusion The fingerprint spectra standard established on the multiple references basis are much more reasonable and useful for the practical quality assurance and validation of Chinese herbals.