目的 研究不同品种和产地化橘红药材黄酮类成分的色谱指纹图谱分析方法。方法 应用 HPL C法以柚皮苷为参比 ,以相对保留时间和峰面积百分比为参数 ,建立 10个样品的 HPLC指纹图谱。结果 标示出 30多个峰 ,且不同品种化橘红 (毛橘红和光橘红 )的图谱存在较大的差异。结论 该方法准确、重现性好 ,适用于化橘红药材的质量控制与鉴定

Objective To compare the UPLC and HPLC fingerprints of flavonoids in Citri Grandis Exocarpium (CGE). Methods The HPLC and UPLC methods for analyzing the fingerprint of 17 samples were established respectively. Results There were 21 mutual peaks selected from UPLC chromatography and 17 mutual peaks selected from HPLC chromatography. The similarity of 17 samples was over 0.9. Conclusion Both the UPLC and HPLC methods could be used for the quality control of CGE. Compared to HPLC, the UPLC method is efficient, quickly, and sensitive.

广东省科技计划项目 (A3 0 10 2 0 10 1)