目的 建立黄芪质量评价的化学模式识别方法。方法 用甲醇回流提取黄芪中皂苷类成分，以氯仿-甲醇-水(65∶30∶10)为展开剂，采用双波长薄层扫描法，在λ_s=390nm，λ_R=590nm下，对18个产地的黄芪样品进行了定量分析，以黄芪甲苷为指标成分，用系统聚类分析对其进行了化学模式识别研究。结果 蒙古黄芪和膜荚黄芪中的黄芪甲苷含量较高，这与《中华人民共和国药典》2000年版将蒙古黄芪和膜荚黄芪列为正品相一致。结论 此方法可用于黄芪质量评价。

Object To establish a method for chemical pattern recognition on the quality assessment of Radix Astragali. Methods The contents of astragaloside in 18 samples of Astragalus Linn. in different species and origins were determinated by dual-wavelength TLCS method. The developing solvent was CHCl₃-MeOH-H₂O ( 65:30:10, ), the UV detection was set at λ_s= 390 nm; λ_R= 590 nm. Astragaloside was regarded as the quality assurance of medicinal Radix Astragalus. Based on the TLCS method, the chemical data were obtained. Hierarchical clustering analyses were applied to the chemical pattern recognition. Results The content of astragaloside in Astragalus mongholicus ( Bge.) Hsiao and A. membranaceus ( Fisch.) Bge. was relatively higher than that in the other samples. This is consistent with the Pharmacopoeia of the People. s Republic of China in which the two sorts of A stragalus Linn. were regarded as goods. Conclusion This method is a pract-icable in the quality assessment of Radix Astragali.

黑龙江省自然科学基金项目(B9803)