目的 建立高效液相色谱法测定灯盏花素片中灯盏花乙素的含量。方法 色谱柱 Diam onsil C₁₈（4.6 mm× 15 0 mm，5 μm)，流动相：乙腈-0.5 %乙酸溶液 （2 2∶ 78)，检测波长：335 nm，柱温：30℃，流速：1.2 m L/min。结果 灯盏花乙素线性范围为 0～ 6.0μg，加样回收率为 10 0.43%，RSD为 0.5 8%。结论 该方法简便、快速、准确，可用于灯盏花素片的质量控制。

Object To establish an HPLC method for the determination of scutellarin in Breviscarpin Tablet. Methods Column: Diamonsil C₁₈ (4.6 mm×150 mm, 5 μm); mobile phase: acetonitrile-0.5% acetate solution (22∶78); detection wavelength was at 335 nm; temperature was at: 30 ℃; flow velocity was 1.2 mL/min. Results The calibration curve showed a good linearity within the range of 0-6.0 μg. The average recovery was 100.43% and RSD was 0.58%. Conclusion The method is convenient, fast and reliable to operate and suitable for the quality control of Breviscarpin Tablet.