目的研究聪圣胶囊对大鼠原代培养皮层神经细胞拟缺血再灌注损伤的影响。方法 采用血清药理学和流式细胞仪的方法 ,观察聪圣胶囊含药血清对缺糖缺氧 (3h)拟缺血再灌注 (0 ,3,6 ,18h)损伤神经细胞乳酸脱氢酶 (L DH)漏出及神经细胞凋亡率的影响。结果 聪圣胶囊含药血清 (2 ,4 ,8g/ kg)可抑制缺糖 -缺氧 3h再灌 (0 ,3,6和 18h)引起的神经细胞内 L DH的释放。降低缺糖 -缺氧 3h再灌 18h导致的凋亡细胞率。结论 聪圣胶囊可减轻缺血性脑损伤。

Object To investigate the profective effects of Congsheng Capsule (CSC) on cultured cerebral cortical neurons after cerebral ischemia reperfusion in order to study the anticerebral ischemia effect of CSC. Methods Drug containing serum was used to study the protective effect of CSC on neurons damaged during hypo glucos and hypoxia (3 h)/reoxygenation (0, 3, 6, 18 h), the demage to neurons was reflected by the increase in activity of lactate dehydrogenase (LDH) released from cells into culture medium and the changes of apoptosic cells which were studied by flow cytometry. Results The serum preparation of CSC (2, 4, 8 g/kg) attenuated neuronal damage during hypo glucos and hypoxia (3 h)/reoxygenation (0, 3, 6, 18 h) by decreased LDH and changes in apoptosic cell numbers. Conclusion CSC has protective effects on cerebral ischemic damage.

“九·五”国家攻关课题 (96-90 6-0 9-0 3)