目的 探讨正品柴胡与同属近似种包括柴胡 Bupleurum chinense、狭叶柴胡 B.scorzonerifolium、竹叶柴胡B.marginatum、小叶黑柴胡 B.smithii var.parvifolium、大叶柴胡 B.longiradiatum 5种柴胡属植物鉴定的分子证据。方法 CTAB法提取柴胡属原植物总 DNA,以随机引物进行非特异扩增。结果 用随机引物 OPA- 1(5'-CAGGCCCTTC- 3'),OPD- 8(5'- GTGTGCCCCA- 3')和 OPD- 11(5'- AGCGCCATTG- 3')能有效地鉴定这 5种柴胡属植物。结论 RAPD法可准确鉴定正品柴胡及其近似种。

Object To define the molecular characters of differentiating Bupleurum chinense DC., B.scorzonerifolium Willd.from their allied species B.marginatum Wall.ex DC., B.smithii Wolff var.parvifolium Shan et Y.Li.and B.longiradiatum Turoz.Methods General DNA was isolated from fresh leaves of Bupleurum L.species by CTAB.The random amplified polymorphic DNA (RAPD) was used to identify them.Results Five Bupleurum L.species can be identified by primer OPA-1(5'-CAGGCCCTTC-3'), OPD-8 (5'-GTGTGCCCCA-3'), OPD-11 (5'-AGCGCCATTG-3').Conclusion The method of RAPD can be used to identify the Bupleurum L.species and its allied species.

国家自然科学基金(39930220)