目的 观察补肾益智方对老年性痴呆 (AD)模型大鼠空间探索学习记忆功能的改善作用。方法 用 15月龄老年 Wistar大鼠 80只,以 D-半乳糖 ip4周加上鹅膏蕈酸 (ibotenic acid,IBO)脑内 Meynert核注射制造 AD模型,随机分成 :AD模型组,双益平治疗对照组,补肾益智方高、低剂量 (12,6 g/ kg· d)治疗组。另加上正常老年组和正常青年组总共设立 6个组别。动物经治疗处理 4周后,应用 Morris水迷宫检测大鼠学习记忆的改善情况。结果 空间探索试验发现,模型组及双益平组在空间探索试验中的指标与正常青年组和正常老年组有显著差异性(P<0.0 1或 P<0.0 5 );补肾益智方高、低剂量组也与模型组和双益平组有显著性差异 (P<0.0 1或 P<0.0 5 )。与模型组比较,正常青年组、正常老年组和补肾益智方高剂量组第 7天撤离平台后大鼠在原平台象限内游泳的时间占整个游泳时间的百分比明显要高 (P<0.0 5 ),而补肾益智方低剂量和双益平组虽然要高些,但差异无显著性 (P>0.0 5 )。结论 补肾益智方能明显改善 AD大鼠空间探索学习记忆功能。

Object To observe the improving effects of BUSHEN YIZHI DECOCTION (BSYZD) on interspace explore learning and memory in rat model with Alzheimer's disease.Methods Eighty 15-month Wistar rats were induced by ip D -galactose for four weeks and injection of basal nucleus of Meynert with ibotenic acid (IBO) to make AD model, then randomly divided into AD model group, Hup-A treated group, BSYZD (high dose, 12 g/kg·d) treated group and BSYZD (low dose, 6 g/kg·d) treated group, and also normal aged and young groups.After treating for four weeks, Morris water maze was used to assess the improvement of rat interspace explore learning and memory.Results In the interspace explore experiment, the significant differences were observed between the model, Hup-A treated groups and normal aged, young groups (P<0.01 or P<0.05); also between the high-, low-dose treated groups of BSYZD and the model, Hup-A treated groups (P<0.01 or P<0.05).Compared with the model group, the percentage of the rat swimming time in platform quadrant was markedly higher in the normal young, aged groups and high-dose treated groups of BSYZD (P<0.05) after departing from the original platform in the 7th day.And there were some in the low-dose treated group of BSYZD and Hup-A treated group, but not markedly (P<0.05).Conclusion BSYZD possesses a certain preventive effects on interspace explore learning and memory in AD rat model.

国家“九五”攻关课题(96-906-09-02)；科技部博士创新课题(98-B-13)