目的 研究外加甲基茉莉酮酸对南方红豆杉 Taxus chinensis var.mairei细胞培养体系氧自由基清除系统中超氧化物歧化酶 (SOD)、过氧化物酶 (POD)和过氧化氢酶 (CAT)酶活的影响规律。方法 酶活分析技术。结果 甲基茉莉酮酸能够诱导南方红豆杉细胞 SOD的活性;对 POD活性的诱导作用极为显著 (约为对照组的 10倍 )且POD活性在培养第 10天达到峰值;在甲基茉莉酮酸作用下 CAT活性先升高后降低 ,即在表现出较强的诱导作用之后又抑制了 CAT的酶活水平。结论 甲基茉莉酮酸的加入刺激细胞产生了防御应答反应 ,诱导了相关的自由基清除酶类 ,其中 POD对甲基茉莉酮酸的信号转导贡献最大。

Object To study the effects of methyl jasmonate(MJ) on SOD, POD and CAT in cell suspension culture of Taxus chinensis var. mairei (Lemee et Lévl.) Cheng et L. K. Fu. Methods SOD, POD and CAT enzymes analysis. Results MJ could induce the activity of SOD and especially of POD (approximately ten times as much as the control), whose activity reached its peak on the 10th day cultured cells. Activity of CAT also increased, but then decreased after the treatment of MJ, which enhanced CAT first then inhibited it. Conclusion Addition of MJ to the cell suspension cultures of T. chinensis var. mairei could cause the cells to elicit the defence response and induce the activities of relative enzymes, in which POD would play the most important role for the signal transduction of MJ.