目的 测定紫锥菊及其复方制剂中菊苣酸的含量。方法 采用 HPLC法,流动相为 4 0 0 mmol/ L 醋酸铵 -甲醇 (95: 5 ),流速为 1mL / m in,检测波长为 330 nm。结果 该法的平均回收率为 99.4 1%,RSD =3.2 3% (n=5 )。结论 该方法可用于紫锥菊药材提取物及其制剂的质量控制

Object To determine the amount of cichoric acid in Enchinacea purpurea and its preparation. Methods By the use of HPLC, mobile phase: 400 mmol/L ammonium acetate methanol (95∶ 5), flow rate: 1 mL/min, detection wavelength: 330 nm. Results The mean recovery of cichoric acid was 99 41%, RSD=3 23% (n=5). Conclusion The method can be applied in the determination of cichoric acid in E. purpurea extract and its preparation with satisfactory results.

科技部国家“十五”攻关项目(201JZY2089)；湖南省科技厅重点项目(01JZY1005)