目的 研究青蒿酯钠诱导人肝癌细胞凋亡作用及探讨青蒿酯钠诱导人肝癌细胞凋亡的分子机制。方法 肝癌细胞(BEL-7402)经药物处理后 ,用荧光显微镜、透射电镜和流式细胞仪分析诱导凋亡作用 ,采用 Westernblot检测 p53、p21和bc1-2。结果 与对照组相比 ,经青蒿酯钠处理后 ,肝癌细胞中p53、p21蛋白表达水平无明显变化 ,而bc1-2蛋白表达水平降低。结论 青蒿酯钠可诱导人肝癌细胞 (BEL-7402)凋亡,其凋亡的分子机制是p53非依赖性的 ,即与p53、p21无关 ,而与凋亡调节基因bc1-2下调有关。

Object To study the apoptosis induced by sodium artesunate (SA) in human liver cancer cells and its molecular mechanism. Methods Induction of the apoptosis was analyzed by fluorescent microscope, transmission electron micrograph and flowly tometry based on drug treated BEL-7402. Expressions of p53, p21 and bc1-2 were determined by Western blot . Results The expression level of p53 and p21 had no change remarkbly , but the expression level of bc1-2 was reduced compared to that of the control group af ter the human liver cancer cells were treated by SA. Conclusion The apoptosis in human liver cancer cells (BEL-7402) may be induced by SA , the molecular mechanism of apoptosis may be p53-independent, that is no relative top53 and p21. Otherwise, it is relative to bc1-2down-regulation.

广东省自然科学基金资助项目(970087)