目的 应用动态比浊法鲎试验定量测定灯盏花素注射液中的细菌内毒素含量.方法 通过对样品中定量添加标准内毒素的干扰试验,检测其回收率应在50%～200%范围.结果 将样品进行1/12稀释可以有效地消除其对鲎试验的干扰.结论 动态比浊法鲎试验可以高效地测定样品中的细菌内毒素含量.

Object To determine the bacterial endotoxin in BREVISCAPINI INJECTION (BI) by kinetic turbidimetric limulus test Methods By spiking the amount of standard endotoxin into BI sample, the interference test was fulfiled to detect the recovery in the range of 50%-200% Results A 12-fold dilution to BI sample is effective to eliminate the interference in limulus test Conclusion The kinetic turbidimetric limulus test provides an efficient way to detect the bacterial endotoxin in BI sample.

广东省重点科技攻关项目(2KM054063)