目的 建立大鼠血浆中丹参酚酸 A的含量测定方法。方法 利用 HPL C,色谱柱 :Hypersil ODS柱 (4.6mm× 2 0 0 mm,10μm),流动相 :乙腈 -水 (1∶ 2 .6 5,甲酸调 pH值至 2 .5 ),流速 :0 .9m L /m in,检测波长 :2 85 nm,以桂皮酸作为内标物。结果 方法回收率在 98.9%～ 10 6 .9%,提取回收率大于 83%,日内、日间精密度均低于8.4 %。结论 该法可作为丹参酚酸 A在大鼠体内药物动力学研究的检测手段。

Object To develop a method for the quantitative determination of salvianolic acid A in rat plasma. Methods HPLC method and Hypersil ODS column (4 6 mm×200 mm, 10 μm) were used, the mobile phase was acetontrile water (1∶2 65, adjusted pH to 2 5 with formic acid), at a flow rate of 0 9 mL/min, the UV detection wavelength was 285 nm. Cinnamic acid was used as internal standard. Results The recovery of the method was in the range of 98 9%～106 9%, the recovery of the extraction was over 83%. The within day precision and the day to day precision were both lower than 8 4%. Conclusion The method is appropriate for the determination of salvianolic acid A when pharmacokinetics of salvianolic acid A is studied in rat plasma.