目的 分析人参四逆汤抗失血性休克作用的提取组分 S- 1和 S- 7的组成成分。方法 结合硅胶色谱柱分离,利用 ESI/MSn、MAL DI- TOF/MS等技术分析鉴定S- 1和 S- 7的化学成分。结果 从人参四逆汤水煎液的抗休克作用的有效组分 S- 7中分析和鉴定了人参皂苷 - Ra₁ 、- Ra₂ 、- Rb₁ 、- Rb₂ 、- Rb₃、- Rc、- Rd、- Re、- Rg₁ 、- Rg₂ 、- Rg₃、- Rf等 12种人参皂苷 ;从有效组分 S- 1中检出下列二萜生物碱成分 :苯甲酰次乌头碱油酸酯 (14 - benzoylhypaconine- 8-linoleate,HAL )、苯甲酰去氧乌头碱油酸酯 (14 - benzoyldeoxyaconine- 8- Oleate,HAO )、苯甲酰次乌头碱棕榈酸酯(14 - benzoylhypaconine- 8- palmitate,HAP)、苯甲酰中乌头碱 (benzoylmesaconitine,BM)、苯甲酰乌头碱 (benzoyla-conitine,BA)、苯甲酰次乌头碱 (benzoylhypaconitine,BH)。结论 首次分析鉴定了人参四逆汤水煎液抗失血性休克作用的提取组分的化学成分。

Object To study the chemical constituents of the extracts S 1 and S 7 in GINSENG SINI TANG, which has the effect of antihemorrhagic shock Methods The constituents of S 1 and S 7 were isolated and purified by silica gel column chromatographic Methods and analyzed by ESI/MS n, MALDI TOF/MS Results The 12 compounds were identified as ginsenosides Ra ₁, Ra ₂, Rb ₁, Rb ₂, Rb ₃, Rc, Rd, Re, Rg ₁, Rg ₂, Rg ₃, Rf from constituent S 7 in GINSENG SINI TANG The six compounds of diterpenoid alkaloid were identified as 14 benzoylhypaconine 8 linoleate (HAL), 14 benzoyldeoxyaconine 8 oleate (HAO), 14 benzoylhypaconine 8 palmitate (HAP), benzoylmesaconitine (BM), benzoylaconitine (BA), benzoylhypaconitine (BH) from constituent S-1 in GINSENG SINI TANG Conclusion All these compounds were obtained from GINSENG SINI TANG and identified for the first time

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