目的研究产地和季节对滇白珠木脂素苷(LG)含量的影响,为开发提供依据。方法高效液相色谱法。色谱柱为KromasilC18(0.4cm×25cm),流动相为甲醇-乙腈-水(30∶5∶65),波长220nm,外标法。结果LG含量在滇白珠内分布规律是:根<茎<叶,只有楚雄林下样品茎高于根。采自海南、广东、云南、贵州、重庆等省市的9个地点的样品中,根中LG含量(mg/g)最高的是海南2.15,其次是贵州2.00,云南楚雄和昆明样品仅1.42和1.44。根中LG含量在9月(果期)以前仅1.438mg/g,比11月低24.4%。结论海南和贵阳样品LG含量最高,但结合生物量考虑,选云南大理和广西金秀的资源更合理。不同季节滇白珠LG含量变化规律是:花期(6～7月)和果期(8～9月)根中含量较低,因此采根应避免花果期。而茎则花果期含量较高,故采茎应选花期。如采收全株8月最适宜。

Object To study the content of lignanglycosides (LG) in Dian Bai Zhu [ Gaultheria leucocarpa var.yunnanensis (Franch.)T.Z.Hsu & R.C.Fang] from different productive area and growth period, so as to provide the basis for development of the plant.Methods T he LG content in Dian Bai Zhu was determined by HPLC with Kromasil C₁₈ column(0.4cm×25cm),mobilephase is methanol-acetonitrile water(30∶5∶65),determinant wavelength is 220 nm.Results The distribution of the LG content in most plants was root> stem > leaf.The only LG content in Chuxiong samplesis stem > root.The comparison of the LG content in nine samples from Hainan,Guangdong,Yunnan,Guizhou and Chong qing suggested that the LG content in the root of the plant from Hainan was the highest,2.15 mg/g;the LG in sample from Guizhou was the second,2.00 mg/g;the LG in samples from Chuxiong and Kunming is the least as 1.42 mg/g and 1.44 mg/g,respectively .The LG content in root was 1.438mg/g before September which was less than that in November by 24.4%.Conclusion The takingresources from Dali,Yunnan and Jinxiu,Guangxi as medicine are appropriately considered their biomass,though the LG content in the plants from Hainan and Guiyang was the highest.The plant root should be harvested after September,because the LG content in root is relat iv ely low before this month; the stem should be har vested during flowering and fruit period(from June to September),the whole plant is best harvested during August.