目的建立麻黄汤煎液中麻黄碱与伪麻黄碱的GC-MS定量方法,测定麻黄汤及麻黄与其它各味中药配伍后构成的组方中麻黄碱与伪麻黄碱的煎出量,并考查其煎出量的变化。方法GC-MS选用特征离子定量法进行测定。结果该法分离效果好,灵敏度高,重现性好。麻黄单煎液中麻黄碱与伪麻黄碱的溶出量较与杏仁合煎液中显著减少,较与桂枝合煎液中显著增多,较与甘草合煎液中无明显变化。结论该法准确、可靠,可作为麻黄汤中麻黄碱与伪麻黄碱的定量方法,并为麻黄方剂药动学研究与治疗药物的进一步监测提供了质控依据。

Object To establish a quantitative method for ephedrine and pseudoephedrine in the aqueous decoctions of MAHUANG TANG (ADMHT) by GC-MS,and to determine the content of the two components in decoctions of the different recipes consisted of Herba Ephedrae ,and other herbs by using the quantitative method will be established, so as to study the effect of compatible medicinal herbs on the release of the two components in Herba Ephedrae to decoctions. Methods The quantitative method by the help of characteristicion was used in GC-MS. Results The content of ephedrine a nd pseudoephedrine in decoction prepared from Herba Ephedrae and Semen Armeniacae Amarum evidently decreases, the content of two components indecoction prepared f rom Herba Ephedrae and Ramulus Cinnamomin evidently increases, the content of two components in decoction prepared from Herba Ephedrae and Radix Glycyrrhizae has no obvious change, as compared with that of two components in decoction from Herba Ephedrae alone. Conclusion The method is good in the aspect of separable effect , sensitivity and reproducibility. The method not only canquantify the two components, but also provide the bases of quality control for the pharmacokinetic study and therapeutic detection of Herba Ephedra recipes.

国家自然科学基金重点资助项目(30030150)