[关键词]
[摘要]
目的 观察红景天、红芪、地黄、枸杞子、沙参和芦荟的多糖成分对T-AK细胞增殖活性和杀瘤活性的增强作用。方法 用抗CD3单抗(CD3McAb)和rIL-2激活诱生T-AK细胞,观察6种植物多糖在诱生、扩增和杀瘤过程中对T-AK细胞增殖活性、IL-2R表达水平以及杀伤Raji细胞和L1210细胞活性的影响。结果 6种植物多糖(1～100μg/mL)单独对T-AK细胞的增殖无明显作用,但在rIL-2和CD3McAb存在下,可不同程度地增强T-AK细胞的增殖能力和杀瘤活性,并增加T-AK细胞IL-2R的表达。结论 红景天、红芪、地黄、枸杞子、沙参和芦荟的多糖成分增强T-AK细胞的增殖和杀伤活性,其作用效应与其促进IL-2R的表达有关
[Key word]
[Abstract]
Object<\b> To study the augmentation of the T activated killer (T AK) cell proliferating and tumor killing activities of the polysaccharide fraction from Rhodiola kirilowii (Regel) Regel, Lycium barbarum L., Hedysarum polybotrys Hand.Mazz., Glehnia littoralis F.Schmidt ex Miq., Rehmannia glutinosa (Gaert.) Libosch.ex Fisch.et Mey.and Aloe barbadensis Mill.in vitro.Methods<\b> The T AK cells were induced by anti CD 3 antibody (CD 3McAb) and rIL 2 from human peripheral blood mononuclear cells (PBMC).The effects of the above six plant polysaccharides (1～100 μg/mL) on the proliferation of T AK cell, the cytotoxicity to Raji cells and L 1210 cells, and the IL 2 receptor (IL 2R) expression in T AK cells were determined.Results<\b> The six polysaccharides alone had no obvious effect on the proliferation of T AK cells.In the presence of rIL 2 and CD 3McAb, they could reinforce the proliferation of T AK cells and its tumor killing activities against Raji cells and L 1210 cells at a different extent, and a 21%～68% increase of IL 2R expression in T AK cells was observed.Conclusion<\b> The plant polysaccharides significantly enhance the proliferation and the tumoricidal activities of T AK cells and the enhancing actions related to the increase of IL 2R expression.
[中图分类号]
[基金项目]
